Designed protein logic to target cells with precise combinations of surface antigens.

ABSTRACT: Precise cell targeting is challenging because most mammalian cell types lack a single surface marker that distinguishes them from other cells. A solution would be to target cells using specific combinations of proteins present on their surfaces. In this study, we design colocalization-dependent protein switches (Co-LOCKR) that perform AND, OR, and NOT Boolean logic operations. These switches activate through a conformational change only when all conditions are met, generating rapid, transcription-independent responses at single-cell resolution within complex cell populations. We implement AND gates to redirect T cell specificity against tumor cells expressing two surface antigens while avoiding off-target recognition of single-antigen cells, and three-input switches that add NOT or OR logi

SUBMITTER: Lajoie MJ 

PROVIDER: S-EPMC8085813 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature