A multiplex antigen microarray for simultaneous IgG and IgM detection against SARS-CoV-2 reveals higher seroprevalence than reported.

ABSTRACT: The surge of SARS-CoV-2 has challenged health systems worldwide and efficient tests to detect viral particles, as well as antibodies generated against them, are needed. Specificity, sensitivity, promptness or scalability are the main parameters to estimate the final performance, but rarely all of them match in a single test. We have developed SCOVAM, a protein microarray with several viral antigens (spike, nucleocapsid, main protease Nsp5) as capturing probes in a fluorescence immunoassay for COVID-19 serological testing. SCOVAM depicts IgG and IgM antibody responses against each of these proteins of 22 individuals in a single microscope slide. It detects specific IgM (0.094 μg ml^-1 ) and IgG (~0.017 μg ml^-1 ) and is scalable and cost-effective. We validated SCOVAM by

SUBMITTER: Ruano-Gallego D 

PROVIDER: S-EPMC8085928 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature