Transcriptional profiling of the stringent response mutant strain E. coli SR reveals enhanced robustness to large-scale conditions.

ABSTRACT: In large-scale fed-batch production processes, microbes are exposed to heterogeneous substrate availability caused by long mixing times. Escherichia coli, the most common industrial host for recombinant protein production, reacts by recurring accumulation of the alarmone ppGpp and energetically wasteful transcriptional strategies. Here, we compare the regulatory responses of the stringent response mutant strain E. coli SR and its parent strain E. coli MG1655 to repeated nutrient starvation in a two-compartment scale-down reactor. Our data show that E. coli SR can withstand these stress conditions without a ppGpp-mediated stress response maintaining fully functional ammonium uptake and biomass formation. Furthermore, E. coli SR exhibited a substantially reduced short-term transcriptional re

SUBMITTER: Ziegler M 

PROVIDER: S-EPMC8085953 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature