Capturing structural changes of the S₁ to S₂ transition of photosystem II using time-resolved serial femtosecond crystallography.

ABSTRACT: Photosystem II (PSII) catalyzes light-induced water oxidation through an S _i -state cycle, leading to the generation of di-oxygen, protons and electrons. Pump-probe time-resolved serial femtosecond crystallography (TR-SFX) has been used to capture structural dynamics of light-sensitive proteins. In this approach, it is crucial to avoid light contamination in the samples when analyzing a particular reaction intermediate. Here, a method for determining a condition that avoids light contamination of the PSII microcrystals while minimizing sample consumption in TR-SFX is described. By swapping the pump and probe pulses with a very short delay between them, the structural changes that occur during the S₁-to-S₂ transition were examined and a boundary of th

SUBMITTER: Li H 

PROVIDER: S-EPMC8086164 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature