Comparative transcriptome analysis reveals key insights into male sterility in Salvia miltiorrhiza Bunge.

ABSTRACT:

Background

Large-scale heterosis breeding depends upon stable, inherited male sterility lines. We accidentally discovered a male sterility line (SW-S) in the F₁progeny of a Salvia miltiorrhiza Bunge from Shandong, China (purple flowers) crossed with a S. miltiorrhiza f. alba from Sichuan, China (white flowers). We sought to provide insights into the pollen development for male sterility in S. miltiorrhiza.

Methods

The phenotypic and cytological features of the SW-S and fertile control SW-F were observed using scanning electron microscopy and paraffin sections to identify the key stage of male sterility. Transcriptome profiles were recorded for anthers at the tetrad stage of SW-S and SW-F using Illumina RNA-Seq.

Results

The paraffin sect

SUBMITTER: Yu Y 

PROVIDER: S-EPMC8086568 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature