Copy number and transcriptome alterations associated with metastatic lesion response to treatment in colorectal cancer.

ABSTRACT:

Background

Therapeutic resistance is the main cause of death in metastatic colorectal cancer. To investigate genomic plasticity, most specifically of metastatic lesions, associated with response to first-line systemic therapy, we collected longitudinal liver metastatic samples and characterized the copy number aberration (CNA) landscape and its effect on the transcriptome.

Methods

Liver metastatic biopsies were collected prior to treatment (pre, n = 97) and when clinical imaging demonstrated therapeutic resistance (post, n = 43). CNAs were inferred from whole exome sequencing and were correlated with both the status of the lesion and overall patient progression-free survival (PFS). We used RNA sequencing data from the same sample set to validate aberrations as well as indepe

SUBMITTER: Gambaro K 

PROVIDER: S-EPMC8087915 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature