Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin.

ABSTRACT:

Background

Ferroptosis is essential to regulate tumor growth and serves as a promising therapeutic target to lung cancer. Ubiquitin-specific protease 35 (USP35) belongs to the deubiquitinases family that is associated with cell proliferation and mitosis. In this research, we aim to elucidate the potential role and molecular basis of USP35 in lung cancer.

Methods

Lung cancer cells were infected with lentiviral vectors to silence or overexpress USP35. Cell viability, colony formation, lipid reactive oxygen species production, intracellular iron metabolism, and other ferroptotic markers were detected. The role of USP35 on ferroptosis and tumor progression were also tested in mouse tumor xenograft models in vivo.

Results

USP35 was abundant in human lung cancer tissues and

SUBMITTER: Tang Z 

PROVIDER: S-EPMC8087931 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature