PINK1-mediated mitophagy maintains pluripotency through optineurin.

ABSTRACT:

Objectives

Dysfunction of autophagy results in accumulation of depolarized mitochondria and breakdown of self-renewal and pluripotency in ESCs. However, the regulators that control how mitochondria are degraded by autophagy for pluripotency regulation remains largely unknown. This study aims to dissect the molecular mechanisms that regulate mitochondrial homeostasis for pluripotency regulation in mouse ESCs.

Materials and methods

Parkin^+/+ and parkin^-/- ESCs were established from E3.5 blastocysts of parkin^+/- x parkin^+/- mating mice. The pink1^-/- , optn^-/- and ndp52^-/- ESCs were generated by CRISPR-Cas9. shRNAs were used for function loss assay of target genes. Mito-Keima, ROS and ATP detection were

SUBMITTER: Wang C 

PROVIDER: S-EPMC8088463 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature