Immunosuppressive effect of mesenchymal stem cells on lung and gut CD8⁺ T cells in lipopolysaccharide-induced acute lung injury in mice.

ABSTRACT:

Objectives

Acute lung injury (ALI) not only affects pulmonary function but also leads to intestinal dysfunction, which in turn contributes to ALI. Mesenchymal stem cell (MSC) transplantation can be a potential strategy in the treatment of ALI. However, the mechanisms of synergistic regulatory effects by MSCs on the lung and intestine in ALI need more in-depth study.

Materials and methods

We evaluated the therapeutic effects of MSCs on the murine model of lipopolysaccharide (LPS)-induced ALI through survival rate, histopathology and bronchoalveolar lavage fluid. Metagenomic sequencing was performed to assess the gut microbiota. The levels of pulmonary and intestinal inflammation and immune response were assessed by analysing cytokine expression and flow cytometry.

Results<

SUBMITTER: Xu Y 

PROVIDER: S-EPMC8088466 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature