RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes.

ABSTRACT:

Objectives

Mammalian spermatogenesis is a biological process of male gamete formation. Gonocytes are the only precursors of spermatogonial stem cells (SSCs) which develop into mature spermatozoa. DDX5 is one of DEAD-box RNA helicases and expresses in male germ cells, suggesting that Ddx5 plays important functions during spermatogenesis. Here, we explore the functions of Ddx5 in regulating the specification of gonocytes.

Materials and methods

Germ cell-specific Ddx5 knockout (Ddx5^-/- ) mice were generated. The morphology of testes and epididymides and fertility in both wild-type and Ddx5^-/- mice were analysed. Single-cell RNA sequencing (scRNA-seq) was used to profile the transcriptome in testes from wild-type and Ddx5^-/- mice at postnatal day

SUBMITTER: Xia Q 

PROVIDER: S-EPMC8088469 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature