Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development.

ABSTRACT:

Background

Human oligodendrocyte precursor cells (hOPCs) are an important source of myelinating cells for cell transplantation to treat demyelinating diseases. Myelin oligodendrocytes develop from migratory and proliferative hOPCs. It is well known that NG2 and A2B5 are important biological markers of hOPCs. However, the functional differences between the cell populations represented by these two biomarkers have not been well studied in depth.

Objective

To study the difference between NG2 and A2B5 cells in the development of human oligodendrocyte progenitor cells.

Methods

Using cell sorting technology, we obtained NG2+/-, A2B5+/- cells. Further research was then conducted via in vitro cell proliferation and migration assays, single-cell sequencing, mRNA sequencing, an

SUBMITTER: Zhou H 

PROVIDER: S-EPMC8088696 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature