Multi-omics reveals age-related differences in the diaphragm response to mechanical ventilation: a pilot study.

ABSTRACT:

Background

Old age is associated with a significantly increased mortality in COVID-19 patients exposed to long-term controlled mechanical ventilation (CMV) and suggested to be due to the hyperinflammatory response associated with the viral infection. However, our understanding of age-related differences in the response to CMV in the absence of a viral infection remains insufficient.

Methods

Young (7-8 months) and old (28-32 months) F344 BN hybrid rats were exposed to the ICU condition for 5 days, i.e., complete immobilization, mechanical ventilation, and extensive monitoring. Transcriptomic (RNA-Seq) and proteomics (Proximity Extension Assay) analyses of the diaphragm and proteomics analysis of plasma were conducted to investigate the molecular differences between young and

SUBMITTER: Lyu Q 

PROVIDER: S-EPMC8089133 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature