Simultaneous directed evolution of coupled enzymes for efficient asymmetric synthesis of l-phosphinothricin.

ABSTRACT: The traditional strategy to improve the efficiency of an entire coupled enzyme system relies on separate direction of the evolution of enzymes involved in their respective enzymatic reactions. This strategy can lead to enhanced single-enzyme catalytic efficiency but may also lead to loss of coordination among enzymes. This study aimed to overcome such shortcomings by executing a directed evolution strategy on multiple enzymes in one combined group that catalyzes the asymmetric biosynthesis of l-phosphinothricin. The genes of a glutamate dehydrogenase from Pseudomonas moorei (PmGluDH) and a glucose dehydrogenase from Exiguobacterium sibiricum (EsGDH), along with other gene parts (promoters, ribosomal binding sites (RBSs), and terminators) were simultaneous

SUBMITTER: Cheng F 

PROVIDER: S-EPMC8090864 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature