Inositol 1,4,5-trisphosphate 3-kinase B promotes Ca²⁺ mobilization and the inflammatory activity of dendritic cells.

ABSTRACT: Innate immune responses to Gram-negative bacteria depend on the recognition of lipopolysaccharide (LPS) by a receptor complex that includes CD14 and TLR4. In dendritic cells (DCs), CD14 enhances the activation not only of TLR4 but also that of the NFAT family of transcription factors, which suppresses cell survival and promotes the production of inflammatory mediators. NFAT activation requires Ca²⁺ mobilization. In DCs, Ca²⁺ mobilization in response to LPS depends on phospholipase C γ2 (PLCγ2), which produces inositol 1,4,5-trisphosphate (IP₃). Here, we showed that the IP₃ receptor 3 (IP₃R3) and ITPKB, a kinase that converts IP₃ to inositol 1,3,4,5-tetrakisphosphate (IP₄), were both necessary for Ca²⁺ m

SUBMITTER: Marongiu L 

PROVIDER: S-EPMC8091591 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature