TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes.

ABSTRACT:

Background

Biological pacemakers consisting of pluripotent stem cell-derived cardiomyocytes are potentially useful for treating bradycardia. However, tachyarrhythmia caused by derived cardiomyocytes themselves is one of main barriers hampering their clinical translation. An in-depth understanding of the mechanisms underlying the spontaneous action potential (a.k.a. automaticity) might provide potential approaches to solve this problem. The aim of this project is to study the role of canonical transient receptor potential isoform 7 (TRPC7) channels in regulating the automaticity of embryonic stem cell-derived cardiomyocytes (ESC-CMs).

Methods and results

By Western blotting, the expression of TRPC7 was found to be increased during the differentiation of mouse ESC-CMs (mESC-CM

SUBMITTER: Liu X 

PROVIDER: S-EPMC8091699 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature