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Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay.

ABSTRACT: Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.

SUBMITTER: Lee DYJ

PROVIDER: S-EPMC8091848 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay.

Lee Darren Y J DYJ Ashcroft Melinda M MM Chow Eric P F EPF Sait Michelle M De Petra Vesna V Tschaepe Marlene M Lange Sigrid S Taiaroa George G Bradshaw Catriona S CS Whiley David M DM Fairley Christopher K CK Howden Benjamin P BP Chen Marcus Y MY Pasricha Shivani S Williamson Deborah A DA

Journal of clinical microbiology 20210420 5

Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N ...[more]

PMID: 33658263

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