Dynamic intracellular mechanical cues facilitate collective signaling responses.

ABSTRACT: Collective behavior emerges in diverse life machineries, e.g., the immune responses to dynamic stimulations. The essential questions that arise here are that whether and how cells in vivo collectively respond to stimulation frequencies higher than their intrinsic natural values, e.g., the acute inflammation conditions. In this work, we systematically studied morphological and signaling responses of population fibroblasts in an interconnected cell monolayer and uncovered that, besides the natural NF-κB oscillation frequency of 1/90 min^-1, collective signaling response emerges in the cell monolayer at 1/20 min^-1 TNF-α input periodicity as well. Using a customized microfluidic device, we independently induced dynamic chemical stimulation and cytoskel

SUBMITTER: Che B 

PROVIDER: S-EPMC8091894 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature