Ontology highlight
ABSTRACT:
SUBMITTER: Lu H
PROVIDER: S-EPMC8092013 | biostudies-literature | 2021 May
REPOSITORIES: biostudies-literature
Lu Hengxing H Liu Jun J Feng Tao T Guo Zihang Z Yin Yunjun Y Gao Fei F Cao Gengsheng G Du Xuguang X Wu Sen S
Genome research 20210401 5
Targeted mutagenesis in model organisms is key for gene functional annotation and biomedical research. Despite technological advances in gene editing by the CRISPR-Cas9 systems, rapid and efficient introduction of site-directed mutations remains a challenge in large animal models. Here, we developed a robust and flexible insertional mutagenesis strategy, homology-independent targeted trapping (HIT-trapping), which is generic and can efficiently target-trap an endogenous gene of interest independ ...[more]