Whole-Genome Transformation Promotes tRNA Anticodon Suppressor Mutations under Stress.

ABSTRACT: tRNAs are encoded by a large gene family, usually with several isogenic tRNAs interacting with the same codon. Mutations in the anticodon region of other tRNAs can overcome specific tRNA deficiencies. Phylogenetic analysis suggests that such mutations have occurred in evolution, but the driving force is unclear. We show that in yeast suppressor mutations in other tRNAs are able to overcome deficiency of the essential TRT2-encoded tRNA^Thr_CGU at high temperature (40°C). Surprisingly, these tRNA suppressor mutations were obtained after whole-genome transformation with DNA from thermotolerant Kluyveromyces marxianus or Ogataea polymorpha strains but from which the mutations did apparently not originate. We suggest that transient presence of donor DNA

SUBMITTER: Deparis Q 

PROVIDER: S-EPMC8092322 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature