Differences in activation of intracellular signaling in primary human retinal endothelial cells between isoforms of VEGFA 165.

ABSTRACT:

Purpose

There are reports that a b-isoform of vascular endothelial growth factor-A 165 (VEGFA₁₆₅b) is predominant in normal human vitreous, switching to the a-isoform (VEGFA₁₆₅a) in the vitreous of some diseased eyes. Although these isoforms appear to have a different ability to activate the VEGF receptor 2 (VEGFR2) in various endothelial cells, the nature of their ability to activate intracellular signaling pathways is not fully characterized, especially in retinal endothelial cells. We determined their activation potential for two key intracellular signaling pathways (MAPK, AKT) over complete dose-response curves and compared potential effects on the expression of several VEGFA₁₆₅ target genes in primary human retinal microvascular endothelial cel

SUBMITTER: Dailey W 

PROVIDER: S-EPMC8092446 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature