A nanobody toolbox targeting dimeric coiled-coil modules for functionalization of designed protein origami structures.

ABSTRACT: Coiled-coil (CC) dimers are widely used in protein design because of their modularity and well-understood sequence-structure relationship. In CC protein origami design, a polypeptide chain is assembled from a defined sequence of CC building segments that determine the self-assembly of protein cages into polyhedral shapes, such as the tetrahedron, triangular prism, or four-sided pyramid. However, a targeted functionalization of the CC modules could significantly expand the versatility of protein origami scaffolds. Here, we describe a panel of single-chain camelid antibodies (nanobodies) directed against different CC modules of a de novo designed protein origami tetrahedron. We show that these nanobodies are able to recognize the same CC modules in different polyhedral contexts, such as isol

SUBMITTER: Majerle A 

PROVIDER: S-EPMC8092592 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature