Investigating variability in microbial community composition in replicate environmental DNA samples down lake sediment cores.

ABSTRACT: Lake sediments are natural archives that accumulate information on biological communities and their surrounding catchments. Paleolimnology has traditionally focussed on identifying fossilized organisms to reconstruct past environments. In the last decade, the application of molecular methodologies has increased in paleolimnological studies, but further research investigating factors such as sample heterogeneity and DNA degradation are required. In the present study we investigated bacterial community heterogeneity (16S rRNA metabarcoding) within depth slices (1-cm width). Sediment cores were collected from three lakes with differing sediment compositions. Samples were collected from a variety of depths which represent a period of time of approximately 1,200 years. Triplicate samples were c

SUBMITTER: Pearman JK 

PROVIDER: S-EPMC8092796 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature