A novel function of African Swine Fever Virus pE66L in inhibition of host translation by the PKR/eIF2α pathway.

ABSTRACT: African swine fever virus (ASFV) is one of the most contagious and lethal viruses infecting pigs. This virus is endemic in many countries and has very recently spread to China, but no licensed vaccines or treatments are currently available. Despite extensive research, the basic question of how ASFV-encoded proteins inhibit host translation remains. Here, we examined how ASFV interfered with host translation and optimized viral gene expression. We found that 14 ASFV proteins inhibited Renilla luciferase (Rluc) activity greater than 5-fold, and the protein with the strongest inhibitory effect was pE66L, which was not previously reported. Combined with bioinformatical analysis and biochemical experiment, we determined that the transmembrane (TM) domain (amino acids 13-34) of pE66L was require

SUBMITTER: Shen Z 

PROVIDER: S-EPMC8092821 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature