Expression of HIV-1 Intron-Containing RNA in Microglia Induces Inflammatory Responses.

ABSTRACT: Chronic neuroinflammation is observed in HIV⁺ individuals on suppressive combination antiretroviral therapy (cART) and is thought to cause HIV-associated neurocognitive disorders. We have recently reported that expression of HIV intron-containing RNA (icRNA) in productively infected monocyte-derived macrophages induces pro-inflammatory responses. Microglia, yolk sac-derived brain-resident tissue macrophages, are the primary HIV-1 infected cell type in the central nervous system (CNS). In this study, we tested the hypothesis that persistent expression of HIV icRNA in primary human microglia induces innate immune activation. We established multiple orthogonal primary human microglia-like cell cultures including peripheral blood monocyte-derived microglia (MDMG) and induced pluripo

SUBMITTER: Akiyama H 

PROVIDER: S-EPMC8092841 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature