Ectopic Tcf1 expression instills a stem-like program in exhausted CD8⁺ T cells to enhance viral and tumor immunity.

ABSTRACT: Exhausted CD8⁺ T (Tex) cells are dysfunctional due to persistent antigen exposure in chronic viral infection and tumor contexts. A stem cell-like Tex (Tex-stem) subset can self-renew and differentiate into terminally exhausted (Tex-term) cells. Here, we show that ectopic Tcf1 expression potently promoted the generation of Tex-stem cells in both a chronic viral infection and preclinical tumor models. Tcf1 overexpression diminished coinhibitory receptor expression and enhanced polycytokine-producing capacity while retaining a heightened responses to checkpoint blockade, leading to enhanced viral and tumor control. Mechanistically, ectopically expressed Tcf1 exploited existing and novel chromatin accessible sites as transcriptional enhancers or repressors and modulated the transcri

SUBMITTER: Shan Q 

PROVIDER: S-EPMC8093427 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature