Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze-Thaw Method.

ABSTRACT: Understanding the structure-function of inclusion bodies (IBs) in the last two decades has led to the development of several mild solubilization buffers for the improved recovery of bioactive proteins. The recently developed freeze-thaw-based inclusion body protein solubilization method has received a great deal of attention due to its simplicity and cost-effectiveness. The present report investigates the reproducibility, efficiency, and plausible mechanism of the freeze-thaw-based IB solubilization. The percentage recovery of functionally active protein species of human growth hormone (hGH) and L-asparaginase from their IBs in Escherichia coli and the quality attributes associated with the freeze-thaw-based solubilization method were analyzed in detail. The overall yield of the pur

SUBMITTER: Singhvi P 

PROVIDER: S-EPMC8093829 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature