Brain-derived neurotrophic factor enhances Bcl-xL expression through protein kinase casein kinase 2-activated and nuclear factor kappa B-mediated pathway in rat hippocampus.

ABSTRACT: Brain-derived neurotrophic factor (BDNF) was shown to produce its neuroprotective effect through extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphatidylinositol-3 kinase (PI3-K) signaling. But whether other pathways also mediate the neuroprotective effect of BDNF is less known. In this study, we found that direct administration of BDNF to rat hippocampal CA1 area dose-dependently increased the mRNA and protein levels of Bcl-xL. BDNF also increased protein kinase casein kinase II (CK2) activity and NF-κB phosphorylation at Ser529 dose-dependently. Further, transfection of the wild-type CK2α DNA to CA1 neurons increased nuclear factor kappa B (NF-κB) phosphorylation and Bcl-xL mRNA expression, whereas transfection of CK2α156A, the catalytically inactive mutant of CK2α, decreased

SUBMITTER: Chao CC 

PROVIDER: S-EPMC8094249 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature