Tetraspanin-7 regulation of L-type voltage-dependent calcium channels controls pancreatic β-cell insulin secretion.

ABSTRACT:

Key points

Tetraspanin (TSPAN) proteins regulate many biological processes, including intracellular calcium (Ca²⁺ ) handling. TSPAN-7 is enriched in pancreatic islet cells; however, the function of islet TSPAN-7 has not been identified. Here, we characterize how β-cell TSPAN-7 regulates Ca²⁺ handling and hormone secretion. We find that TSPAN-7 reduces β-cell glucose-stimulated Ca²⁺ entry, slows Ca²⁺ oscillation frequency and decreases glucose-stimulated insulin secretion. TSPAN-7 controls β-cell function through a direct interaction with L-type voltage-dependent Ca²⁺ channels (Ca_V 1.2 and Ca_V 1.3), which reduces channel Ca²⁺ conductance. TSPAN-7 slows activation of Ca_V 1.2 and acceler

SUBMITTER: Dickerson MT 

PROVIDER: S-EPMC8095317 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature