Transcriptome-scale spatial gene expression in the human dorsolateral prefrontal cortex.

ABSTRACT: We used the 10x Genomics Visium platform to define the spatial topography of gene expression in the six-layered human dorsolateral prefrontal cortex. We identified extensive layer-enriched expression signatures and refined associations to previous laminar markers. We overlaid our laminar expression signatures on large-scale single nucleus RNA-sequencing data, enhancing spatial annotation of expression-driven clusters. By integrating neuropsychiatric disorder gene sets, we showed differential layer-enriched expression of genes associated with schizophrenia and autism spectrum disorder, highlighting the clinical relevance of spatially defined expression. We then developed a data-driven framework to define unsupervised clusters in spatial transcriptomics data, which can be applied to other ti

SUBMITTER: Maynard KR 

PROVIDER: S-EPMC8095368 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature