The conserved translation factor LepA is required for optimal synthesis of a porin family in Mycobacterium smegmatis.

ABSTRACT: The recalcitrance of mycobacteria to antibiotic therapy is in part due to its ability to build proteins into a multi-layer cell wall. Proper synthesis of both cell wall constituents and associated proteins is crucial to maintaining cell integrity, and intimately tied to antibiotic susceptibility. How mycobacteria properly synthesize the membrane-associated proteome, however, remains poorly understood. Recently, we found that loss of lepA in Mycobacterium smegmatis (Msm) altered tolerance to rifampin, a drug that targets a non-ribosomal cellular process. LepA is a ribosome-associated GTPase found in bacteria, mitochondria, and chloroplasts, yet its physiological contribution to cellular processes is not clear. To uncover the determinants of LepA-mediated drug tolerance, we cha

SUBMITTER: Fishbein SRS 

PROVIDER: S-EPMC8095456 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature