Protein dosage of the lldPRD operon is correlated with RNase E-dependent mRNA processing.

ABSTRACT: The ability of Escherichia coli to grow on L-lactate as a sole carbon source depends on the expression of the lldPRD operon. A striking feature of this operon is that the transcriptional regulator (LldR) encoding gene is located between the permease (LldP) and the dehydrogenase (LldD) encoding genes. In this study we report that dosage of the LldP, LldR, and LldD proteins is not modulated on the transcriptional level. Instead, modulation of protein dosage is primarily correlated with RNase E-dependent mRNA processing events that take place within the lldR mRNA, leading to the immediate inactivation of lldR, to differential segmental stabilities of the resulting cleavage products, and to differences in the translation efficiencies of the three cistrons. A model for the processing events con

SUBMITTER: Angel-Lerma LE 

PROVIDER: S-EPMC8095457 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature