INT-Hi-C reveals distinct chromatin architecture in endosperm and leaf tissues of Arabidopsis.

ABSTRACT: Higher-order chromatin structure undergoes striking changes in response to various developmental and environmental signals, causing distinct cell types to adopt specific chromatin organization. High throughput chromatin conformation capture (Hi-C) allows studying higher-order chromatin structure; however, this technique requires substantial amounts of starting material, which has limited the establishment of cell type-specific higher-order chromatin structure in plants. To overcome this limitation, we established a protocol that is applicable to a limited amount of nuclei by combining the INTACT (isolation of nuclei tagged in specific cell types) method and Hi-C (INT-Hi-C). Using this INT-Hi-C protocol, we generated Hi-C data from INTACT purified endosperm and leaf nuclei. Our INT-Hi-C dat

SUBMITTER: Yadav VK 

PROVIDER: S-EPMC8096224 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature