Complement-activated human endothelial cells stimulate increased polyfunctionality in alloreactive T cells.

ABSTRACT: Antibody-mediated deposition of complement membrane attack complexes (MACs) on IFN-γ-primed human endothelial cells (ECs) triggers autocrine/paracrine IL-1β-mediated EC activation and IL-15 transpresentation to alloreactive effector memory T cells (T_EM ), changes that enable ECs to increase T cell proliferation and cytokine release. Here, we report the use of single-cell microchip 32-plex proteomics to more deeply assess the functionality of the activated T cells and dependence upon EC-derived signals. Compared to control ECs, MAC-activated human ECs increase both the frequency and degree of polyfunctionality among both CD4⁺ and CD8⁺ -proliferated T_EM , assessed as secreted proteins. IFN-γ and TNF-α remain the predominant cytokines made by allore

SUBMITTER: Xie CB 

PROVIDER: S-EPMC8096653 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature