Single molecule tracking and analysis framework including theory-predicted parameter settings.

ABSTRACT: Imaging, tracking and analyzing individual biomolecules in living systems is a powerful technology to obtain quantitative kinetic and spatial information such as reaction rates, diffusion coefficients and localization maps. Common tracking tools often operate on single movies and require additional manual steps to analyze whole data sets or to compare different experimental conditions. We report a fast and comprehensive single molecule tracking and analysis framework (TrackIt) to simultaneously process several multi-movie data sets. A user-friendly GUI offers convenient tracking visualization, multiple state-of-the-art analysis procedures, display of results, and data im- and export at different levels to utilize external software tools. We applied our framework to quantify dissociation ra

SUBMITTER: Kuhn T 

PROVIDER: S-EPMC8096815 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature