Serial profiling of cell-free DNA and nucleosome histone modifications in cell cultures.

ABSTRACT: Recent advances in basic research have unveiled several strategies for improving the sensitivity and specificity of cell-free DNA (cfDNA) based assays, which is a prerequisite for broadening its clinical use. Included among these strategies is leveraging knowledge of both the biogenesis and physico-chemical properties of cfDNA towards the identification of better disease-defining features and optimization of methods. While good progress has been made on this front, much of cfDNA biology remains uncharted. Here, we correlated serial measurements of cfDNA size, concentration and nucleosome histone modifications with various cellular parameters, including cell growth rate, viability, apoptosis, necrosis, and cell cycle phase in three different cell lines. Collectively, the picture emerged tha

SUBMITTER: Ungerer V 

PROVIDER: S-EPMC8096822 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature