Analysis of tRNA ^Cys processing under salt stress in Bacillus subtilis spore outgrowth using RNA sequencing data.

ABSTRACT: Background: In spore-forming bacteria, the molecular mechanisms of accumulation of transfer RNA (tRNA) during sporulation must be a priority as tRNAs play an essential role in protein synthesis during spore germination and outgrowth. However, tRNA processing has not been extensively studied in these conditions, and knowledge of these mechanisms is important to understand long-term stress survival.    Methods:To gain further insight into tRNA processing during spore germination and outgrowth, the expression of the single copy tRNA ^Cys gene was analyzed in the presence and absence of 1.2 M NaCl in Bacillus subtilis using RNA-Seq data obtained from the Gene Expression Omnibus (GEO) database. The CLC Genomics work bench 12.0.2 (CLC Bio, Aarhus, Denmark, https://

SUBMITTER: Arvizu Hernandez I 

PROVIDER: S-EPMC8097732 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature