Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus.

ABSTRACT:

Background

Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression and gene silencing in plants. The capacity of MDMV for both gene expression and gene silencing were examined.

Results

Infectious clones of an Ohio isolate of MDMV, MDMV OH5, were obtained, and engineered for gene expression only, and for simultaneous marker gene expression and virus-induced gene silencing (VIGS) of three endogenous maize target genes. Single gene expression in single insertion constructs and simultaneous expression of green fluorescent protein (GFP) and silencing of three maize genes in a double insertion construct was demonstrated. Constructs with GFP

SUBMITTER: Xie W 

PROVIDER: S-EPMC8097858 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature