Nanopattern surface improves cultured human myotube maturation.

ABSTRACT:

Background

In vitro maturation of human primary myoblasts using 2D culture remains a challenging process and leads to immature fibers with poor internal organization and function. This would however represent a valuable system to study muscle physiology or pathophysiology from patient myoblasts, at a single-cell level.

Methods

Human primary myoblasts were cultured on 800-nm wide striated surface between two layers of Matrigel, and in a media supplemented with an inhibitor of TGFβ receptor. Gene expression, immunofluorescence, and Ca²⁺ measurements upon electrical stimulations were performed at various time points during maturation to assess the organization and function of the myotubes.

Results

We show that after 10 days in culture, myotubes display numerou

SUBMITTER: Brunetti J 

PROVIDER: S-EPMC8097894 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature