Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography.

ABSTRACT: Serial synchrotron crystallography (SSX) is enabling the efficient use of small crystals for structure-function studies of biomolecules and for drug discovery. An integrated SSX system has been developed comprising ultralow background-scatter sample holders suitable for room and cryogenic temperature crystallographic data collection, a sample-loading station and a humid `gloveless' glovebox. The sample holders incorporate thin-film supports with a variety of designs optimized for different crystal-loading challenges. These holders facilitate the dispersion of crystals and the removal of excess liquid, can be cooled at extremely high rates, generate little background scatter, allow data collection over >90° of oscillation without obstruction or the risk of generating saturating Bragg peaks,

SUBMITTER: Illava G 

PROVIDER: S-EPMC8098472 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature