PhyloPrimer: a taxon-specific oligonucleotide design platform.

ABSTRACT: Many environmental and biomedical biomonitoring and detection studies aim to explore the presence of specific organisms or gene functionalities in microbiome samples. In such cases, when the study hypotheses can be answered with the exploration of a small number of genes, a targeted PCR-approach is appropriate. However, due to the complexity of environmental microbial communities, the design of specific primers is challenging and can lead to non-specific results. We designed PhyloPrimer, the first user-friendly platform to semi-automate the design of taxon-specific oligos (i.e., PCR primers) for a gene of interest. The main strength of PhyloPrimer is the ability to retrieve and align GenBank gene sequences matching the user's input, and to explore their relationships through an online dyna

SUBMITTER: Varliero G 

PROVIDER: S-EPMC8098674 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature