Dynamic organization of cortical actin filaments during the ooplasmic segregation of ascidian Ciona eggs.

ABSTRACT: Spatial reorganization of cytoplasm in zygotic cells is critically important for establishing the body plans of many animal species. In ascidian zygotes, maternal determinants (mRNAs) are first transported to the vegetal pole a few minutes after fertilization and then to the future posterior side of the zygotes in a later phase of cytoplasmic reorganization, before the first cell division. Here, by using a novel fluorescence polarization microscope that reports the position and the orientation of fluorescently labeled proteins in living cells, we mapped the local alignments and the time-dependent changes of cortical actin networks in Ciona eggs. The initial cytoplasmic reorganization started with the contraction of vegetal hemisphere approximately 20 s after the fertilization-induce

SUBMITTER: Ishii H 

PROVIDER: S-EPMC8098833 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature