Ontology highlight
ABSTRACT:
SUBMITTER: Kwon S
PROVIDER: S-EPMC8099091 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Kwon Sunghark S Chun Hye Lin HL Ha Hyun Ji HJ Lee So Yeon SY Park Hyun Ho HH
PloS one 20210505 5
During the glyoxylate cycle, isocitrate lyases (ICLs) catalyze the lysis of isocitrate to glyoxylate and succinate. Itaconate has been reported to inhibit an ICL from Mycobacterium tuberculosis (tbICL). To elucidate the molecular mechanism of ICL inhibition, we determined the crystal structure of tbICL in complex with itaconate. Unexpectedly, succinate and itaconate were found to bind to the respective active sites in the dimeric form of tbICL. Our structure revealed the active site architecture ...[more]