Recent Insights into the Structure and Function of Mycobacterial Membrane Proteins Facilitated by Cryo-EM.

ABSTRACT: Mycobacterium tuberculosis (Mtb) is one of the deadliest pathogens encountered by humanity. Over the decades, its characteristic membrane organization and composition have been understood. However, there is still limited structural information and mechanistic understanding of the constituent membrane proteins critical for drug discovery pipelines. Recent advances in single-particle cryo-electron microscopy and cryo-electron tomography have provided the much-needed impetus towards structure determination of several vital Mtb membrane proteins whose structures were inaccessible via X-ray crystallography and NMR. Important insights into membrane composition and organization have been gained via a combination of electron tomography and biochemical and biophysical assays. In addition, till the

SUBMITTER: Bendre AD 

PROVIDER: S-EPMC8099146 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature