Disruption of entire <i>Cables2</i> locus leads to embryonic lethality by diminished <i>Rps21</i> gene expression and enhanced p53 pathway.

ABSTRACT: In vivo function of CDK5 and Abl enzyme substrate 2 (Cables2), belonging to the Cables protein family, is unknown. Here, we found that targeted disruption of the entire Cables2 locus (Cables2d) caused growth retardation and enhanced apoptosis at the gastrulation stage and then induced embryonic lethality in mice. Comparative transcriptome analysis revealed disruption of Cables2, 50% down-regulation of Rps21 abutting on the Cables2 locus, and up-regulation of p53-target genes in Cables2d gastrulas. We further revealed the lethality phenotype in Rps21-deleted mice and unexpectedly, the exon 1-deleted Cables2 mice survived. Interestingly, chimeric mice derived from Cables2d ESCs carrying exogenous Cables2 and tetraploid wil

SUBMITTER: Dinh TTH 

PROVIDER: S-EPMC8099427 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature