Enhanced fusidic acid transdermal delivery achieved by newly isolated and optimized Bacillus cereus Keratinase.

ABSTRACT: The expanding interest in bioremediation of poorly degradable wastes has led to the discovery of many microbial enzymes capable of degrading recalcitrant substances such as keratinaceous wastes that are produced in vast quantities on daily basis. Such enzymes don't only work as a bioremediation tool but also have multiple beneficial applications. Hence, environmental samples were collected from sewage water, soils, animal bodies and feces in order to isolate keratinase producing organisms. Keratinolytic isolates were isolated from sewage water; soils; animal bodies; animal feces, and identified both traditionally and molecularly through 16S-rRNA sequencing to be Bacillus cereus strain. Produced keratinase was purified by centrifugation, ammonium sulfate precipitation, and HPLC, then

SUBMITTER: Shalaby MM 

PROVIDER: S-EPMC8099499 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature