Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation.

ABSTRACT: The plant plasma membrane (PM) is an essential barrier between the cell and the external environment, controlling signal perception and transmission. It consists of an asymmetrical lipid bilayer made up of three different lipid classes: sphingolipids, sterols, and phospholipids. The glycosyl inositol phosphoryl ceramides (GIPCs), representing up to 40% of total sphingolipids, are assumed to be almost exclusively in the outer leaflet of the PM. However, their biological role and properties are poorly defined. In this study, we investigated the role of GIPCs in membrane organization. Because GIPCs are not commercially available, we developed a protocol to extract and isolate GIPC-enriched fractions from eudicots (cauliflower and tobacco) and monocots (leek and rice). Lipidomic analysis confi

SUBMITTER: Mamode Cassim A 

PROVIDER: S-EPMC8099651 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature