Boosting mitochondria activity by silencing MCJ overcomes cholestasis-induced liver injury.

ABSTRACT:

Background & aims

Mitochondria are the major organelles for the formation of reactive oxygen species (ROS) in the cell, and mitochondrial dysfunction has been described as a key factor in the pathogenesis of cholestatic liver disease. The methylation-controlled J-protein (MCJ) is a mitochondrial protein that interacts with and represses the function of complex I of the electron transport chain. The relevance of MCJ in the pathology of cholestasis has not yet been explored.

Methods

We studied the relationship between MCJ and cholestasis-induced liver injury in liver biopsies from patients with chronic cholestatic liver diseases, and in livers and primary hepatocytes obtained from WT and MCJ-KO mice. Bile duct ligation (BDL) was used as an animal model of cholestasis, and prim

SUBMITTER: Iruzubieta P 

PROVIDER: S-EPMC8099785 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature