Pre-treatment of blood samples reveal normal blood hypocretin/orexin signal in narcolepsy type 1.

ABSTRACT: The hypocretin/orexin system regulates arousal through central nervous system mechanisms and plays an important role in sleep, wakefulness and energy homeostasis. It is unclear whether hypocretin peptides are also present in blood due to difficulties in measuring reliable and reproducible levels of the peptides in blood samples. Lack of hypocretin signalling causes the sleep disorder narcolepsy type 1, and low concentration of cerebrospinal fluid hypocretin-1/orexin-A peptide is a hallmark of the disease. This measurement has high diagnostic value, but performing a lumbar puncture is not without discomfort and possible complications for the patient. A blood-based test to assess hypocretin-1 deficiency would therefore be of obvious benefit. We here demonstrate that heating plasma or serum s

SUBMITTER: Ægidius HM 

PROVIDER: S-EPMC8100001 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature