Fluorescent quenching probes based SAA 1 genotyping with a fully automated system.

ABSTRACT:

Objective

The aim of the present study is to develop and validate a reliable and simple application for genotyping serum amyloid A1 (SAA1).

Methods

The specific nested PCR was performed to amplify a product of SAA1 gene. Two quenching probes (QPs) were designed for detecting two single nucleotide polymorphism (SNP) sites, rs1136743(C/T) and rs1136747(C/T) respectively for SAA1 genotypes. The specific nested PCR and QPs of SAA1 genotying was introduced into a fully automated genotyping system (I-densy, ARKRAY, Inc.), which enables the genotyping of SAA1 from whole blood.

Results

Six genotypes of SAA1 (α^+/+, β^+/+, γ^+/+, αβ, αγ and βγ) could be determined by monitoring the fluorescence intensity o

SUBMITTER: Zhang J 

PROVIDER: S-EPMC8100075 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature