Trichosporon asahii and Trichosporon inkin Biofilms Produce Antifungal-Tolerant Persister Cells.

ABSTRACT: Persister cells are metabolically inactive dormant cells that lie within microbial biofilms. They are phenotypic variants highly tolerant to antimicrobials and, therefore, associated with recalcitrant infections. In the present study, we investigated if Trichosporon asahii and T. inkin are able to produce persister cells. Trichosporon spp. are ubiquitous fungi, commonly found as commensals of the human skin and gut microbiota, and have been increasingly reported as agents of fungemia in immunocompromised patients. Biofilms derived from clinical strains of T asahii (n=5) and T. inkin (n=7) were formed in flat-bottomed microtiter plates and incubated at 35°C for 48 h, treated with 100 μg/ml amphotericin B (AMB) and incubated at 35°C for additional 24 h. Bio

SUBMITTER: Cordeiro RA 

PROVIDER: S-EPMC8100310 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature